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人结直肠腺癌细胞HCT-15
  【编号】:HCT-15

  【产品名称】:人结直肠腺癌细胞HCT-15

  【规格】:株

  【用途】:仅供研究之用

  HCT-15人结直肠腺癌细胞

  描述:DNA fingerprinting证据表明,这株细胞和DLD-1 (ATCC CCL-221) 来源于同一个人;但同工酶及细胞染色体组型分析仍存疑问。 细胞呈CSAp阴性(CSAp-)。 角蛋白免疫过氧化物酶染色阳性。
  动物种别:人
  性别:男
  组织来源:结直肠腺癌
  形态:上皮细胞
  培养基和添加剂:RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
  供应限制:A。仅供研究之用。
  REFERENCE:Schroy PC , et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 8625092 Journal of Interferon & Cytokine Research 24:219,2004. Brattain MG , et al. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 41: 1751-1756, 1981. PubMed: 7214343 Sun L , et al. Autocrine transforming growth factor-beta 1 and beta 2 expression is increased by cell crowding and quiescence in colon carcinoma cells. Exp. Cell Res. 214: 215-224, 1994. PubMed: 8082724 Santoro IM , Groden J . Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479 Brattain MG , et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756 Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064 Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486 Wang R , et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887 MOLECULAR THERAPY 11(4):531,2005www.atcc360.com
  中文名称:人结肠癌细胞
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