梭菌蛋白酶 来源于溶组织梭菌(测序级), ≥50 units/mg dry weight
| 【编号】：128676 【产品名称】：梭菌蛋白酶 来源于溶组织梭菌(测序级), ≥50 units/mg dry weight 【规格】：10ug 【用途】：   梭菌蛋白酶 来源于溶组织梭菌(测序级), ≥50 units/mg dry weight
Product Name： Clostripain from Clostridium histolyticum(Sequencing Grade)
CAS号：9028-00-6
介绍： Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.
用途： A protease that cleaves proteins on the carboxyl bond of argininewww.atcc360.com
生化和生理学机理： Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.
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