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DNA酶I 来源于牛胰腺, ≥2,000 Kunitz units/mg dry weight
  【编号】:128592

  【产品名称】:DNA酶I 来源于牛胰腺, ≥2,000 Kunitz units/mg dry weight

  【规格】:25mg

  【用途】:

  DNA酶I 来源于牛胰腺, ≥2,000 Kunitz units/mg dry weight

  Product Name: Deoxyribonuclease I from bovine pancreas
  别名:脱氧核糖核酸酶 I;脱氧核糖核酸 5′-寡核苷酸-水解酶
  CAS号:9003-98-9
  介绍: Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
  用途: 用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
  贮存: 储存温度2-8℃
  生化和生理学机理: DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity.
上一篇:DNA酶I 来源于牛胰腺(无Ribonuclease & Protease), ≥2,000 units/mg dry weight 下一篇:DNA酶I 来源于牛胰腺(过滤), ≥2,000 units/mg dry weight



    
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