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小鼠胚胎成纤维细胞C3H/10T1/2, Clone 8
  【编号】:C3H/10T1/2, Clone 8

  【产品名称】:小鼠胚胎成纤维细胞C3H/10T1/2, Clone 8

  【规格】:株

  【用途】:仅供研究之用

  C3H/10T1/2, Clone 8小鼠胚胎成纤维细胞

  描述:该细胞系是由C. Reznikoff等(1972)从C3H系小鼠胚胎细胞分离。此细胞对过度长满的细胞有丝分裂抑制非常敏感,在同源小鼠中不产生肿瘤,无自然转化的背景,也不含明显内源性转化鼠类白血病或肉瘤病毒。
  动物种别:小鼠
  性别:未知
  组织来源:胚胎
  形态:成纤维细胞
  培养基和添加剂:MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
  供应限制:A。仅供研究之用。
  REFERENCE: Reznikoff CA , et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800 Terzaghi M , Little JB . Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940 Mondal S , Heidelberger C . Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242 Smith GJ , et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183 Rapp UR , et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, www.atcc360.com。1975. PubMed: 165619 Reznikoff CA , et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355 Jain MK , et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. 
  中文名称:小鼠胚胎成纤维细胞
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