白介素12（人类，rDNA衍生）世卫组织参考试剂

  【编号】：NIBSC95/544

  【名称】：白介素12（人类，rDNA衍生）世卫组织参考试剂

  【规格】：1个

  【价格】：2600元

　　白介素12（人类，rDNA衍生）世卫组织参考试剂

　　编号：NIBSC95/544

　　英文：Interleukin-12 (Human, rDNA derived) WHO Reference Reagent

　　标准类型：国际参考试剂

　　规格：1个

　　描述：1. INTENDED USE

This Standard is the primary biological standard for IL-12.

2. CAUTION

This preparation is not for administration to humans or animals in

the human food chain.

The preparation contains material of human origin, and either the final

product or the source materials, from which it is derived, have been

tested and found negative for HBsAg, anti-HIV and HCV RNA. As with

all materials of biological origin, this preparation should be regarded as

potentially hazardous to health. It should be used and discarded

according to your own laboratory's safety procedures. Such safety

procedures should include the wearing of protective gloves and

avoiding the generation of aerosols. Care should be exercised in

opening ampoules or vials, to avoid cuts.

3. UNITAGE

The assigned potency is 10,000 Units of biological activity per ampoule.

4. CONTENTS

Country of origin of biological material: United Kingdom.

Each ampoule contains a freeze dried residue comprising, under an

atmosphere of nitrogen:

IL-12 approximately 1 microgram

Potassium phosphate buffer

1.0 mg trehalose

2.0 mg human serum albumin

The IL-12 protein was expressed in CHO cells.

5. STORAGE

For economy of use, it is recommended that the solution be sub

divided into several small aliquots and stored at -40ºC or below.

Avoid repeated thawing/freezing. Unopened ampoules should be

stored at -20ºC.

Please note: because of the inherent stability of lyophilized

material, NIBSC may ship these materials at ambient temperature.

6. DIRECTIONS FOR OPENING

DIN ampoules have an 'easy-open' coloured stress point, where the

narrow ampoule stem joins the wider ampoule body. Various types of

ampoule breaker are available commercially. To open the ampoule,

tap the ampoule gently to collect material at the bottom (labelled) end

and follow manufactures instructions provided with the ampoule

breaker.

7. USE OF MATERIAL

No attempt should be made to weigh out any portion of the freeze-dried

material prior to reconstitution

Dissolve the total contents of the ampoule in 0.5ml of sterile distilled

water. Rinse the ampoule with about 0.4ml of sterile distilled water and

make up the total volume to 1.0ml with distilled water. This solution will

contain IL-12 at a concentration of 10000 Units/ml. Use carrier protein

where extensive dilution is required.

8. STABILITY

Reference materials are held at NIBSC within assured, temperaturecontrolled

storage facilities and they should be stored on receipt as

indicated on the label. It is the policy of WHO not to assign an expirydate to their international reference materials. Accelerated degradation

studies have indicated that this material is suitably stable, when stored at

-20ºC or below, for the assigned values to remain valid until the material

is withdrawn or replaced. These studies have also shown that the

material is suitably stable for shipment at ambient temperature without

any effect on the assigned values. Once reconstituted, diluted or

aliquoted, users should determine the stability of the material according

to their own method of preparation, storage and use. Users who have

data supporting any deterioration in the characteristics of any reference

preparation are encouraged to contact NIBSC.

9. REFERENCES

This standard was produced under WHO guidelines cited in the WHO

Technical Reports Series, No. 800, 1990, Annex 4.

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