白介素2可溶性受体（人类，rDNA衍生）

  【编号】：NIBSC97/600

  【名称】：白介素2可溶性受体（人类，rDNA衍生）

  【规格】：个

  【价格】：2200元

　　白介素2可溶性受体（人类，rDNA衍生）

　　编号：NIBSC97/600

　　英文：Interleukin-2 Soluble Receptor (human, rDNA derived)

　　标准类型：非WHO参考资料

　　规格：1个

　　描述：1. INTENDED USE

2. CAUTION

This preparation is not for administration to humans or animals in

the human food chain.

The preparation contains material of human origin, and either the final

product or the source materials, from which it is derived, have been

tested and found negative for HBsAg, anti-HIV and HCV RNA. As with

all materials of biological origin, this preparation should be regarded as

potentially hazardous to health. It should be used and discarded

according to your own laboratory's safety procedures. Such safety

procedures should include the wearing of protective gloves and

avoiding the generation of aerosols. Care should be exercised in

opening ampoules or vials, to avoid cuts.

3. UNITAGE

The assigned potency is 1000 units of immunological activity per

ampoule.

4. CONTENTS

Country of origin of biological material: United Kingdom.

Each ampoule contains a freeze dried residue comprising, under an

atmosphere of nitrogen:

IL-2sr approximately 30 - 100 ng

9.0 mg sodium chloride

1.0 mg trehalose

2.0 mg human serum albumin

The IL-2sr protein was expressed in CHO cells.

NOTE: Due to the variation in estimates provided by contributors to an

international collaborative study of the mass of IL-2sr contained within the

ampoules, a unitage has been applied to the contents and a range

provided for the mass content. It is strongly recommended that all

immunoassays are calibrated in this unitage.

5. STORAGE

For economy of use, it is recommended that the solution be sub divided

into several small aliquots and stored at -40ºC or below. Avoid repeated

thawing/freezing. Unopened ampoules should be stored at -20ºC.

Please note: because of the inherent stability of lyophilized material,

NIBSC may ship these materials at ambient temperature.

6. DIRECTIONS FOR OPENING

DIN ampoules have an 'easy-open' coloured stress point, where the

narrow ampoule stem joins the wider ampoule body. Various types of

ampoule breaker are available commercially. To open the ampoule,

tap the ampoule gently to collect material at the bottom (labelled) end

and follow manufactures instructions provided with the ampoule

breaker.

7. USE OF MATERIAL

No attempt should be made to weigh out any portion of the freeze-dried

material prior to reconstitution

Dissolve the total contents of the ampoule in 0.5ml of sterile distilled

water. Rinse the ampoule with about 0.4ml of sterile distilled water and

make up the total volume to 1.0ml with distilled water. This solution will contain IL-2 sr at a concentration of 1000 units/ml. Use carrier protein where

extensive dilution is required.

8. STABILITY

Reference materials are held at NIBSC within assured, temperaturecontrolled

storage facilities and they should be stored on receipt as

indicated on the label. An arbitrary unitage was assigned to this

preparation at the time of production without confirmation of long term

stability. Evidence from similar materials prepared by an equivalent

process indicates that long term stability is likely to be maintained and the

material is suitably stable for shipment at ambient temperature without

any effect on the assigned values. Once reconstituted, diluted or

aliquoted, users should determine the stability of the material according

to their own method of preparation, storage and use. Users who have

data supporting any deterioration in the characteristics of any reference

preparation are encouraged to contact NIBSC.

9. REFERENCES

This standard was produced under WHO guidelines cited in the WHO

Technical Reports Series, No. 800, 1990, Annex 4.

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