脊髓灰质炎病毒1型（Sabin）的MAPREC分析100％480-A，525-C DNA（1st国际参考制

  【编号】：NIBSC00/410

  【名称】：脊髓灰质炎病毒1型（Sabin）的MAPREC分析100％480-A，525-C DNA（1st国际参考制

  【规格】：个

  【价格】：2600元

　　脊髓灰质炎病毒1型（Sabin）的MAPREC分析100％480-A，525-C DNA（1st国际参考制剂）

　　编号：NIBSC00/410

　　英文：MAPREC assay of poliovirus type 1 (Sabin).100% 480-A, 525-C DNA(1st International Reference Preparation)

　　标准类型：国际参考制剂

　　规格：1个

Customer Notes 00/416 - MAPREC assay of poliovirus type 1 (Sabin) Low Mutant Virus Reference(1st International Reference Preparation) 00/418 - MAPREC assay of poliovirus type 1 (Sabin) (1st International Standard) 00/422 - MAPREC assay of poliovirus type 1 High(Sabin) Mutant Virus Reference(1st International Reference Preparation)

　　描述：1. INTENDED USE

This control DNA for the MAPREC assay of polio virus type 1 (Sabin) is

intended to be used as a validation sample for the assay. It is used to

control the performance of the restriction enzymes. Dde I and Nci I, for

example should digest this sample completely but assay conditions may

reduce the efficiency of digestion. Conditions of restriction enzyme

digestion should be optimized with this sample and monitored for

consistency thereafter.

2. CAUTION

This preparation is not for administration to humans or animals in

the human food chain.

The material is not of human or bovine origin.

As with all materials of biological origin, this preparation should be

regarded as potentially hazardous to health. It should be used and

discarded according to your own laboratory's safety procedures. Such

safety procedures should include the wearing of protective gloves and

avoiding the generation of aerosols. Care should be exercised in

opening ampoules or vials, to avoid cuts.

3. UNITAGE

Preparation 00/410 is assigned a nominal value of 100% 480-A, 100%

525-C.

4. CONTENTS

Country of origin of biological material: USA.

Each DIN 58-377 ampoule contains the freeze-dried residue of cloned

DNA that spans nucleotides 445-561 of type 1 poliovirus (Sabin) RNA.

The DNA was prepared at a nominal concentration of 0.01µg/ml in TE

buffer pH7.5 (10mM Tris-HCl and 1mM EDTA) and 0.1% w/v lactose was

added as a bulking agent prior to freeze-drying.

5. STORAGE

Unopened ampoules should be stored at -20°C or below.

Rehydrate preparation 00/410 in 0.1ml of distilled water. Aliquot the

rehydrated material in 10µl volumes and store at -70°C.

Please note: because of the inherent stability of lyophilized

material, NIBSC may ship these materials at ambient temperature.

6. DIRECTIONS FOR OPENING

DIN ampoules have an 'easy-open' coloured stress point, where the

narrow ampoule stem joins the wider ampoule body. Various types of

ampoule breaker are available commercially. To open the ampoule,

tap the ampoule gently to collect material at the bottom (labelled) end

and follow manufactures instructions provided with the ampoule

breaker.

7. USE OF MATERIAL

No attempt should be made to weigh out any portion of the freeze-dried

material prior to reconstitution

A Standard Operating Procedure for the MAPREC assay is available

from; Chief, Biologicals, World Health Organization. This procedure

requires that an aliquot of 00/410 is tested in each MAPREC assay and

used to validate the test.

8. STABILITY

Reference materials are held at NIBSC within assured, temperaturecontrolled

storage facilities. Reference Materials should be stored on

receipt as indicated on the label.

NIBSC follows the policy of WHO with respect to its reference materials.

9. REFERENCES

1. WHO (2002) WHO Technical Report Series, 904, 31-91.

2. European Pharmacopoeia, 6th Edition (2009) Vaccines for human use,

Poliomyelitis vaccine oral 04/2008:0215.

3. WHO /BS/09.2103. WHO Technical Report Series.

10. ACKNOWLEDGEMENTS

We thank the participants of the WHO collaborative study to evaluate the

Poliovirus type1 MAPREC assay

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脊髓灰质炎病毒1型（Sabin）的MAPREC分析100％480-A，525-C DNA（1st国际参考制剂）

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脊髓灰质炎病毒1型（Sabin）的MAPREC分析100％480-A，525-C DNA（1st国际参考制剂）

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